Moisture resistant microbial penetration tester

microbe penetration tester for medical protective clothing

a large number of examples have shown that bacteria can penetrate shielding materials with dry organic or inorganic particles, such as bacteria-carrying dander or cleaning clothing, and as bacteria-carrying particles penetrate packaging materials during storage. This instrument can be used to measure the bacterial penetration performance of dry particles

1 in the range of resistance to human dander size.

tests were carried out on specimens fixed on a container. Among these, five containers carried subtilisin talc, and one containing uninfected talc served as a control. A petri dish was inserted at the bottom of each container close to the bottom of the specimen.

the device supporting the container oscillates by a gas ball oscillator, which penetrates the specimen and drops all the talc onto the petri dish, which is then taken out and cultured.

for growing colony count.

technical specifications

1. Test box: black inner wall, volume: 0.5m3, built-in row fan and lighting; 2. Air source of the instrument: 0.8mpa compressed air;

3. Noise: about 90dB; 4. Touch screen operation; 5. Instrument size: 1000mm´690mm´1390mm; 6. Instrument weight: 85kg

7. Power supply: 220V.

configuration list

this instrument should be carefully unpacked and checked. If the instrument is damaged or lost, please contact our customer service department.

when you open the packing, please check the following materials (as per the actual packing list) :

1. 1 main engine;

3.

4. Manual 1

5. Optional:

6. Air storage tank

2. Test standard

dry microorganism penetration tester tests samples in accordance with ISO 22612, YY/T 5056.5 test standard. 3. Composition and use of the instrument

instrument is completed in strict accordance with the standard drawings, fully conform to and can achieve the test content required by the standard.

the instrument includes:

(1) pneumatic ball oscillator: the external air source passes through the pneumatic ball oscillator, through the corresponding adjustment and correction to maintain the vibration frequency at the target frequency. It generates 20,800 vibrations per minute with a force of 650N.

(2) compressed air flowmeter: it is mainly used to measure the flow which produces 20800(347Hz) vibration frequency per minute.

(3) test containers: the instrument has 6 stainless steel test containers with a metal piston on the top. The metal piston can be inserted through the center hole of the cover to reach 10mm below the cover, and ensure that the specimen does not relax after insertion. At the same time, each container has a slot at the bottom of the attachment, which is used to insert the petri dish.

(4) marble slab: 400mm*400mm area and 10mm thick marble as the operation platform of the whole instrument.

(5) fixed plate: a stainless steel plate with 6 holes, which is fixed on the stone plate with a jig, used for fixing and installing the test container

(6) rubber support: with 4 rubber supports, used for adjusting the level, fixing the position of the instrument and preventing movement. < / p > < p > < / p > < p > & have spent

test

1. In these containers, five containers containing subtilisin talc served as the test group, and one container containing uncontaminated talc served as the control group. Insert a petri dish at the bottom of each container close to the bottom of the specimen.

supporting the container equipment by a gas ball oscillator to make it oscillate, through the specimen talc all fell on the petri dish, take out the petri dish and culture.

was used to count the number of growing colonies. 2. Equipment debugging:

to ensure that the dry microorganism penetration tester can be operated. Adjust the level

(1) adjust the level

adjust the 4 rubber supports according to the level to make the instrument in the horizontal position. (2) adjust the frequency of the gas ball oscillator

(2) adjust the gas ball oscillator frequency

to reach 20800 vibrations per minute by adjusting the gas ball oscillator frequency. < / p > < p > 3. The sample preparation: < / p > < p > (1) cutting 12 under test < / p > < p > 12 pieces of sample size is 200 mm * 200 mm.

(2) sterilization treatment

sterilized the cut specimens and each test container.

(3) fixed test container

fixed the test container on the fixed plate through rubber ring, so that it is tightly connected to the fixed plate, with the bottom resting on the SLATE.

(4) talc powder preparation

prepare talc powder containing AATCC 9372 bacillus subtilis spore, the concentration should be about 108CFU/g, the preparation process refer to YY/T 5056.5-2009.

4.

use aseptic operation method to take out the specimens and put them on the mouth of each test container.

by pushing down the piston, the lid was fixed on the container, so that the specimen was fixed under a controlled degree of relaxation. Less than /p> less than p> take off the piston. 0.5g±0.1g of talc contaminated with bacteria was added to the specimen through the piston mouth, and the sixth container as the control was added with uncontaminated talc. Seal the piston mouth with a film.

each container with a small plastic bag.

the petri dishes are inserted through the slit at the bottom of each container. Close the mouth with sticky tape.

vibrates at a frequency of 20800 vibrations per minute for 30min.

remove plastic bags and tape.

was inserted into the lid of the petri dish through a narrow mouth, the petri dish was taken out and cultured at 35℃ for 24h.

counts the colonies formed. The valid sample should be the control plate with a reading of 0. Otherwise, the test should be suspended due to external pollution.

repeat the above experimental steps.

zui tested 2 groups and calculated arithmetic mean value for 10 valid results as the final result of zui.

test end

when the test is over, ensure that the data has been recorded, adjust the voltage regulator knob on the panel to 0 scale. Turn off the instrument intake, turn on the exhaust fan and lighting, and clean the surface of each sample box.

5. Maintenance

keep the instrument dry

to ensure normal use of the instrument and extend its service life, please keep the instrument dry. And in a relatively dry environment to avoid water droplets splash or condensation, so as not to damage non-water-resistant devices. If the instrument is not used for a long time, please disconnect the power supply and cut off the air source.

component damage

instrument internal starting oscillator is a vulnerable component, reaching the service life, the accuracy of oscillation frequency will be reduced. It is recommended that new parts be replaced at this time.

marble at the same time if there is damage, please timely replace the same specification accessories.

microbial safety

to prevent contamination of the experimenter and the test environment, the experimenter shall have the operational basis for microbial culture. Follow the laboratory practice.